In Vitro Quorum Quenching Activity of Eleusine indica Crude Ethanolic Extract against Pseudomonas aeruginosa and Serratia marcescens

Introduction. Nosocomial contaminants such as Pseudomonas aeruginosa and Serratia marcescens are increasingly developing resistance to many antibiotics. One of the promising alternatives that may complement, if not substitute, use antibiotics is quorum quenching, process interfering with chemical signals mediate communication between microorganisms. Eleusine indica, a ubiquitous grass used traditionally treat infections, has been shown contain metabolites, fatty acid derivatives p-coumaric acid, capable quenching. To date, there no study on quenching activity E. indica. Objectives. This aimed determine in vitro crude ethanolic extract indica leaves against selected quorum-sensing regulated virulence factors P. S. marcescens. Methodology. were collected, washed, air-dried, homogenized. Following extraction rotary evaporation, was screened for antimicrobial through disk diffusion test broth microdilution assay. The measured swarming motility assay, while pigment inhibition assays. assays conducted triplicates, analysis variance (ANOVA) performed identify differences among treatment groups. Results. Disk revealed zones formed both marcescens  varying concentrations up 200 mg/mL extract. Likewise, MIC determined be >200 mg/mL. However, it extract, at 50 mg/mL, statistically significant (p<0.05) aeruginosa, 71.6% effective reducing area bacteria compared cinnamaldehyde. observed when tested production by marcescens. Conclusion. In this study, found but marcescens. compounds will identified further studies conceivably an adjunct therapy infections coating agents medical devices.